Fig. 2

Time course of 2,3-BDO fermentation using pure glycerol and crude glycerol by K. oxytoca M1. a Fed-batch fermentation with pure glycerol. b Fed-batch fermentation with crude glycerol. The following symbols were used: dry cell weight (unfilled triangle), residual glycerol (unfilled square), 2,3-BDO (filled circle), 1,3-PDO (filled square), and lactic acid (filled triangle)