Fig. 2

Endoglucanase activity (units/mg of protein) from fresh leaves of transgenic TcEG1 plants. a Endoglucanase activity measurement using carboxymethyl cellulose (CMC) as substrate at pH 12.0 on TcEG1 extracted from fresh leaves. Bars represent mean values of three replicates ± standard error for each transgenic event. Bars represented by different letters are significantly different as calculated by LSD (p ≤ 0.05). b Gradient pH measurement of endoglucanase activity of TcEG1 extracted from fresh leaves of transgenic event Tc-1. Data points represent mean values of three replicates ± standard error. Data points represented by different letters are significantly different as calculated by LSD (p ≤ 0.05)