Fig. 7

Synergy experiment between the cellulase active LPMO CelS2 and cellulase TwCel5 showing the boost in hydrolytic activity against Avicel. A TwCel5CAT, TwCel5CBM or CelS2 each 1 µM were incubated alone or in combination for different time durations, all with 1 mM ascorbate included in the reaction mixture. B Reactions conducted from the oxidised products of avicel by CelS2 after 48 h treatment were incubated with the TwCel5 variants for 18 h; “control” refers to a reaction in which no enzyme was added. Values obtained are means and standard deviation derived from triplicate