Fig. 3
Glycerol, acetate and acetaldehyde production (A) and PRK and CbbM-derived peptide abundance determined by LC–MS and displayed as the percentage of PRK or CbbM protein in the total protein pool (B) in anaerobic chemostat cultures of IME324 (reference strain lacking PRK/RuBisCO bypass), IMX1489 (Δgpd2, non-ox PPP↑, pDAN1-prk, 15 × cbbm, GroES/GroEL), IMX2736 (Δgpd2, non-ox PPP↑, pDAN1-prk, 2 × cbbm, GroES/GroEL), IMX2701 (Δgpd2, non-ox PPP↑, pDAN1-prk-CLN2PEST, 2 × cbbm, GroES/GroEL), IMX2593 (Δgpd2, non-ox PPP↑, pDAN1-prk-19aa, 2 × cbbm, GroES/GroEL) and IMX2608 (Δgpd2, non-ox PPP↑, pANB1-prk, 2 × cbbm, GroES/GroEL) at a dilution rate of 0.05 h−1. Values represent means and individual values of measurements on independent steady-state duplicate cultures. Electron recoveries were calculated as balances of degree of reduction of substrates and products [21]