Fig. 4

Recombinant DNA constructs used in energy cane transformation. A Expression cassettes of WRI1 from Sorghum bicolor controlled by the Brachypodium distachyon elongation factor 1-α promoter (pBdEF1α) and Panicum virgatum ubiquitin (tPvUbiII) terminator, and the selectable marker gene neomycin phosphotransferase (nptII) driven by the maize ubiquitin promoter (pZmUbi) and Sorghum bicolor heat shock protein terminator (tSbHSP). B Expression cassettes for OLE1, obtained from Sesamum indicum, was under the control of the Brachypodium distachyon ubiquitin promoter (pBdUbi10) and Nicotiana benthamiana actin 3ʹ UTR terminator (tNbACT3), and DGAT1 from Tropaeolum majus, without intron was cloned under the control of the Panicum virgatum ubiquitin promoter (pPvUbiII) and Nicotiana tabacum extensin terminator (tNtEU). C Expression cassettes for OLE1, obtained from Sesamum indicum, was under the control of the Brachypodium distachyon ubiquitin promoter (pBdUbi10) and Nicotiana benthamiana actin 3ʹ UTR terminator (tNbACT3), and DGAT1 from Tropaeolum majus, including a 110-bp-long intron from the predicted Sorghum bicolor 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 was cloned under the control of the Panicum virgatum ubiquitin promoter (pPvUbiII) and Nicotiana tabacum extensin terminator (tNtEU) W: no intron. In: intron