Fig. 5

Generation of transgenic energy cane. A Leaf whorl cross-sections were used as explants. B Callus induced from leaf whorl cross-section was used as the target for biolistic gene transfer. C Calli regenerating on medium with geneticin for selection of nptII expression. D Regenerated plants before transferring to soil. E Transgenic energy cane lines growing in greenhouse. Bar = 5 mm