Fig. 1

Schematic representation of the sucrose utilization pathway and metabolic engineering strategies used in strain SCUT27. A Sucrose PTS system, B sucrose permease system, and C secreted sucrose 6-phosphate hydrolase system. Solid lines indicate metabolic pathway (the heterogenous are in red) or phosphoryl group transfer. Red cross represents the gens deletion and red arrows show the upregulated expression of the genes. The source of sucrose-utilizing genes was shown in left. G1P, glucose 1-phosphate; G6P, glucose 6-phosphate; FBP, fructose 1,6-bisphosphate; PMS, permease system