Fig. 4

Analysis of the potential inhibitory effect of FFCA/FDCA on CglAlcOx activity for HMFCA oxidation. A Measurement of the oxidation capacity of CglAlcOx using mixed substrates containing 2.5Â mM FFCA and 2.5Â mM HMFCA. B Verification of the role of BpLac in alleviating the inhibitory effect of FFCA on CglAlcOx in cascade reactions containing 2.5Â mM FFCA and 2.5Â mM HMFCA C. Measurement of the oxidation capacity of CglAlcOx using mixed substrates containing 2.5Â mM FDCA and 2.5Â mM HMFCA. D Analysis of the oxidation capacity of mixed enzymes (BpLac and CglAlcOx) to the mixed chemicals (2.5Â mM HMFCA and 2.5Â mM FDCA)