Fig. 2

Analysis of changes in P. pastoris cell wall permeability after knocking out ΔPAS-0305. Assessment of whole-cell permeability via a intracellular Nile red fluorescence measurement; b intracellular propidium iodide fluorescence measurement; and c measurement of CMCase activity of heterologously HgCel5A. d Comparison of P. pastoris extracellular protein. d1, SDS-PAGE. d2, extracellular protein concentration analysis