Fig. 5

A schematic illustration of the strategies used in the current study to construct a cellodextrin- and xylose-fermenting Y. lipolytica for lipid overproduction. The pathway for cellodextrin phosphorolysis is marked with light yellow box, while xylose assimilation pathway is marked with pink box. Lipid pathway engineering is indicated in cyan box. PPP pathway overexpression to enhance NADPH supply is marked with gray box. The heterologous genes are written in red and the native genes are given in blue. The interrupted genes are marked with a red deletion line. GPD1 glycerol-3-phosphate dehydrogenase, DGA2 acyl-CoA:diacylglycerol acyl transferase, MFE1 multifunctional beta-oxidation enzyme hydratase-dehydrogenase-epimerase, PEX10 peroxisome biogenesis, TGL TAG lipase