Fig. 6

Accumulation of Green Fluorescent Protein (GFP) aggregates in the presence of isobutanol. A Z. mobilis strain, engineered to express GFP, was grown in minimal media supplemented with either ethanol (at concentrations of 0.3, 0.6, or 0.8Â M) or isobutanol (at concentrations of 0.10 or 0.15Â M). Fluorescence microscopy analysis revealed the emergence of GFP aggregates exclusively in the media containing isobutanol