Fig. 2

Gene real-time expression levels at 6 h and 72 h under lignin and starvation Collected Ct values of lignin, glucose and starvation group of different genes at 6 h and 72 h, then analyzed and calculated the relative expression levels of corresponding target genes in different samples. Finally, the expression level of genes in the glucose group was set as the control value 1, the relative expression levels of the lignin and starvation group were calculated, respectively, when glucose was used as the control, and then Log₂E logarithm was used to draw the heat map. Each experimental group was conducted in triplicate