Fig. 1

Overview of the steps in the reconstruction of the genome-scale metabolic model of A. tubingensis. The process of reconstruction of the genome-scale metabolic model of A. tubingensis involved three major steps: draft model reconstruction, manual curation and expansion, and compartmentalisation of the reactions in the model. The draft model reconstruction was based on KEGG annotation of A. niger CBS 513.88 as well as A. tubingensis annotation to generate a homology-based draft model. The two draft models were then merged. The model was further expanded based on KEGG database, phenotypic microarray data (Biolog plates) and published literature on secondary metabolites. This was followed by compartmentalisation of the reactions using CELLO and WoLF-PSORT, addition of transport reactions and assigning the GPR as well as Boolean rules