Fig. 4

Effect of pH, H₂O₂ and temperature on Ape-MnP1 and Cst-MnP1 stability. A pH stability as residual activity after 24 h incubation in 100 mM Britton–Robinson buffer (pH 2–9) at 25 °C. B H₂O₂ stability assayed by incubating the enzymes with 500 and 1000 equivalents of H₂O₂ in 10 mM sodium acetate pH 5.5 at 25 °C, and further measurement of their residual activities. C Thermal effect on MnP activity after incubation in 10 mM sodium acetate, pH 5.5, for 10 min. D Effect of temperature on protein denaturation measured by CD at 222 nm. Residual activities in pH and H₂O₂ stability analyses were measured with 10 mM ABTS and 0.3 mM H₂O₂ in 100 mM sodium tartrate pH 3.0–3.5. Residual activities in thermal stability analyses were determined towards 10 mM MnSO₄ in 100 mM sodium tartrate pH 4.0–5.0. T_m and T₅₀ are provided. Means and standard deviations are shown