Fig. 5

Dated phylogenetic tree of 336 POD sequences from 42 Agaricales, Polyporales, Amylocorticiales and Russulales species. Mean ages (and 95% highest posterior density, hpd, intervals) of the most recent common ancestors of four MnP subfamilies (in Ma) are indicated adjacent to the nodes. The color of the nodes refers to the MnP subfamily to which the reconstructed ancestor belongs, and the color of the branches indicates the type of the next ancestor (or current enzyme) in the tree: grey, generic peroxidase (GP); light orange, short MnP (MnP-s); dark orange, long MnP (MnP-l); red, versatile peroxidase (VP); pink, atypical VP (VPa); purple, lignin peroxidase (LiP); dark blue, MnP-ED; green, MnP-DDQ; light blue, MnP-DED; brown, NPOD. The position of Cst-MnP1 and Ape-MnP1 is shown inside boxes of the color of the subfamily they belong to. The name of the species and the number of PODs of each (sub)family (in parentheses) are shown at the tips of the branches