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Fig. 7 | Biotechnology for Biofuels and Bioproducts

Fig. 7

From: Structure–function characterization of two enzymes from novel subfamilies of manganese peroxidases secreted by the lignocellulose-degrading Agaricales fungi Agrocybe pediades and Cyathus striatus

Fig. 7

Molecular mass distribution of enzyme-treated and control softwood, LSS (A, B); and hardwood, LSH (C, D) lignosulfonates. Lignosulfonate treatments with 1 µM native enzymes (green lines) and lignosulfonate treatments with 1 µM native enzymes and 4 mM MnSO4 (orange lines), carried out by incubating 12 g·L-1 lignosulfonates in 50 mM sodium tartrate pH 5.0 for 24 h in the presence of 10 mM H2O2, were analyzed in a Superdex-75 column using 0.15 M NaOH/NaCl as mobile phase, and detection at 280 nm. Control lignosulfonates incubated with 4 mM MnSO4 are indicated with a blue line

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