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Table 3 Comparison of yoghurt production processes for low lactose content

From: Simultaneous fermentation and enzymatic biocatalysis—a useful process option?

Literature

Substrate

Process, conditions

Lactose conversion, β-galactosidase*

Final lactose content/g (100 g)−1

Lactic acid quanti-fication

Process time

[72]

Cow milk

Fermentation, 41 °C

35%

4.4

Titratable acidity, 1.1%

8 h

Simultaneous, 41 °C

99%, A. oryzae, 0.9 U mL−1, 1 g L−1

0.1

Titratable acidity, 1.0%

8 h

[54]

Cow milk

Fermentation, 39 °C

12.6%

3.9

n.d

7 h

Hydrolysis 4–6 °C, subsequent fermentation 39 °C

98.5%, Bacillus licheniformis, 18.1 U mL−1, 3.3 g L−1

0.3

n.d

4 h + 6.5 h

Simultaneous 39 °C

84.2%, B. licheniformis, 17.3 U mL−1, 3.3 g L−1

0.7

n.d

6 h

[54]

Goat milk

Fermentation, 39 °C

14.0%

3.9

n.d

7 h

Hydrolysis 4–6 °C, subsequent fermentation 39 °C

96.5%, B. licheniformis, 18.1 U mL−1, 3.3 g L−1

0.2

n.d

4 h + 6.5 h

Simultaneous 39 °C

82.7%, B. licheniformis, 17.3 U mL−1, 3.3 g L−1

0.8

n.d

6 h

[31]

Camel milk

Fermentation, 42 °C

27.0%

3.3

Titratable acidity, 0.7%

7.5 h

Hydrolysis 40 °C, 150 rpm, subsequent fermentation, 42 °C

90.0%, K

lactis, 2.6 U mL−1, 0.5 g L−1

0.4

Titratable acidity, 0.8%

5 h + 4.8 h

Simultaneous, 42 °C

71.2%, K

lactis, 2.6 U mL−1, 0.5 g L−1

1.3

Titratable acidity, 0.8%

4.6 h

[77]

Bulk bovine milk

Fermentation, 42 °C

18.7%

3.8

HPLC, 6.9 mg g−1

3.9 h

Simultaneous, 42 °C

91.4%, K. lactis, 0.4 g L−1, activity unknown,

0.4

HPLC, 6.5 mg g−1

4.3 h

[45]

Milk powder

Fermentation, 43 °C

19.8%,

4.6

n.d

3.9 h

Simultaneous, 43 °C

97.9%, K. lactis and

A. niger, 0.5 g L−1 activity unknown,

0.1

n.d

4.3 h

  1. *Enzyme activity calculated from manufacturer information, enzyme activity related to the yoghurt volume
  2. n.d.: not detected