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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Metabolic engineering of Escherichia coli for the production of 5-aminolevulinic acid based on combined metabolic pathway modification and reporter-guided mutant selection (RGMS)

Fig. 4

Results of RGMS. A Relative Fluorescent Strength of RGMS mutants. (Y axis: Relative Fluorescent Strength). B Approximately 6 ~ 10 mutants which showed strong fluorescence were selected to measure ALA production without replications. (Y axis: relative ALA titers). C 2 ~ 3 mutants which have high titers of ALA production were isolated and remeasured ALA accumulation with three replications. Y axis: relative ALA titers. The control was set as strains which harbored pSC-LA-eamA

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