Metabolic engineering of Escherichia coli for the production of 5-aminolevulinic acid based on combined metabolic pathway modification and reporter-guided mutant selection (RGMS)

Table 1 ALA production in recombinant E. coli expressing various related genes

Strains	Character	Expressed genes	ALA concentration (mg/L)
BWT7-RSA	E. coli BW25113-T7	hemA, hemL, eamA	765.94 ± 25.16
A2-RSA	E. coli A2 (E. coli BW25113-T7 ΔaceB, ΔdppA, ΔhemF, ΔgalR int ppc with J23119, ΔpoxB int glk with J23119)	hemA, hemL, eamA, hemARS	2056.11 ± 59.48
BWT7-LAA	E. coli BW25113-T7	hemA, hemL, eamA	718.05 ± 9.17
BWT7-LAA(C)	E. coli BW25113-T7	hemA, hemL, eamA(C)	804.17 ± 13.21
BWT7-LAA(C1)	E. coli BW25113-T7	hemA, hemL, eamA(C1)	846.68 ± 12.16
A2-RSA-C1	E. coli A2	hemA, hemL, eamA(C1), hemARS	2471.33 ± 60.31
A2-ASK	E. coli A2	hemA, hemL, eamA(C1), hemARS, katE, sodB	2703.75 ± 93.59
108-ASK	E. coli 108 (E. coli A2 ΔPhemB, Int::J23108)	hemA, hemL, eamA(C1), hemARS, katE, sodB	3267.09 ± 105.48
111-ASK	E. coli 111 (E. coli A2 ΔPhemB, Int::J23111)	hemA, hemL, eamA(C1), hemARS, katE, sodB	3434.19 ± 137.88
116-ASK	E. coli 116 (E. coli A2 ΔPhemB, Int::J23116)	hemA, hemL, eamA(C1), hemARS, katE, sodB	2245.29 ± 123.34

A 1% (v/v) inoculum from an overnight culture for 12 h was used. IPTG was added in 4 h when OD₆₀₀ reached 0.7. Samples were taken and measured until 24 h. 10 g/L glucose was added initially as sole carbon source. Glycine (2 g/L) as substrate for C4 pathway was added as indicated. The results are the average of three individual experiments

ISSN: 2731-3654