Table 2 Batch fermentation of ALA in E. coli A2-ASK, 108-ASK and 111-ASK in a 5 L fermenter
Strain | Expressed genes | Cell biomass (OD600) | ALA concentration (g/L) |
|---|---|---|---|
E. coli A2 | hemA, hemL, eamA(C1), hemARS, katE, sodB | 32.76 ± 0.44 | 13.62 ± 0.62 |
E. coli 108 (E. coli A2 ΔPhemB, Int::J23108) | hemA, hemL, eamA(C1), hemARS, katE, sodB | 32.20 ± 0.53 | 19.02 ± 0.38 |
E. coli 111 (E. coli A2 ΔPhemB, Int::J23111) | hemA, hemL, eamA(C1), hemARS, katE, sodB | 31.90 ± 0.78 | 10.73 ± 0.36 |
Strain | ALA production (g) | Glucose consumption (g) | ALA production rate (g/g) |
|---|---|---|---|
E. coli A2 | 34.50 ± 1.55 | 222.24 ± 2.00 | 0.155 ± 0.007 |
E. coli 108 (E. coli A2 ΔPhemB, Int::J23108) | 49.00 ± 0.89 | 235.70 ± 0.71 | 0.208 ± 0.003 |
E. coli 111 (E. coli A2 ΔPhemB, Int::J23111) | 26.76 ± 0.98 | 196.95 ± 2.75 | 0.136 ± 0.007 |