Fig. 1

E. coli central carbon metabolism engineered for isopropanol production on acetate. AckA acetate kinase, Pta phosphate acetyltransferase, Acs acetyl-coenzyme A synthetase, GltA citrate synthase, AcnA/AcnB aconitate hydratase A/B, ICDH isocitrate dehydrogenase, SucA 2-oxoglutarate dehydrogenase E1 component, SucB dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, SucC succinate–CoA ligase [ADP-forming] subunit beta, SucD succinate-CoA ligase [ADP-forming] subunit alpha, SdhCDAB succinate dehydrogenase, FumABCDE fumarate hydratase, Mdh malate dehydrogenase, AceA isocitrate lyase, AceB malate synthase, MaeA NAD-dependent malic enzyme, MaeB NADP-dependent malic enzyme, Pck Phosphoenolpyruvate carboxykinase, Ppc phosphoenolpyruvate carboxylase, PykA pyruvate kinase II, PykF pyruvate kinase I, PDH pyruvate dehydrogenase, PoxB pyruvate dehydrogenase (ubiquinone), Thl thiolase, AtoDA acetate CoA-transferase, Adc acetoacetate decarboxylase, Adh(P) NADPH-dependent secondary alcohol dehydrogenase