Fig. 8

The elimination of C4R2I5 improved the tolerance and production of 2-PEÂ in yeast. A Differentially expressed genes in the transcriptome of the GS115 and PDR12 strains were knocked out under 2-PE conditions. B The dilution spot-plate assay showed that differential gene knockout improved K. phaffii tolerance to 2-PE. C Flask fermentation experiment of engineered strains