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Table 3 Calculation of redox and carbon balances of resting cells of B. propionicifaciens

From: Propionate production by Bacteroidia gut bacteria and its dependence on substrate concentrations differs among species

  

A

B

C

Substrate/products [mM]

[H]

GAP

[mM]

[mM]

Glucose

    

 1

Glycolysis→GAP

16.6

 

33.2

 2

oxPPP→GAP

–

–

–

 3

GAP→PEP

33.2

33.2

 

Products

 4

pyruvate

1.2

0.0

1.2

 5

succinate

1.3

−2.7

1.3

 6

propionate

13.3

−26.6

13.3

 7

acetate

7.9

7.9

7.9

 8

acetate + formate

0.7

0.0

0.7

 9

lactate

0.0

0.0

0.0

 

GAP products [mM]

 

24.4

 

GAP yield [%]

 

74

 

[Htotal] [mM]

24.4

 
 

[Hproducts] [mM]

−21.3

 
 

[H] yield [%]

 

87.1

 
  1. Product concentrations after 24 h of incubation were utilized for calculations according to Table 3. NADH + H+, NADPH + H+ and Fdred. were simplified as [H]. Oxidation reactions of the substrate received a positive value, whereas reduction reactions received a negative value. GAPproducts: sum of C4—C9. GAP yield: GAPproducts *100/A3. [Htotal]: reduction equivalents produced from GAP products + B2. [Hproducts]: sum of B4—B9, the reduction equivalents used for the formation of the fermentation products. [H] yield: [Hproducts] *100/[Htotal]
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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