Fig. 4

RNA-Seq analysis of WT, OEGlr1, OEAn03 and OEYap1. A Delineates the expression profiles of common secreted proteins in A. niger, such as glaA, amyA, and aamA. B Delineates the expression profiles of ROS-producing genes in A. niger. C Intracellular ROS levels in A. niger strains with the knockout of Glr1, An03 and Yap1. D Glucoamylase activity of A. niger strains with the knockout of Glr1, An03 and Yap1. E Total secretory protein levels in A. niger strains with the knockout of Glr1, An03 and Yap1. F Shows the enrichment in OEYap1 and OEGlr1, with the vertical axis denoting function annotation information and the horizontal axis representing the Rich factor. The Rich factor is determined by dividing the number of differentially expressed genes ascribed to a certain function by the total number of genes annotated to that function. The color intensity of each dot is proportional to the Q-value magnitude; a lower Q-value produces a color closer to red. Furthermore, the dot size represents the number of differentially expressed genes for each function. To ensure clarity, only the top 30 Gene Ontology (GO) terms with the highest enrichment degree were plotted