Table 3 Ethanol-based biomanufacturing

Bacteria

E. coli

Introducing exogenous ADH gene from Gluconobacter thailandicus

Co-culture, 2% ethanol

Produce NADH for xylitol production

\

[70]

E. coli

Expressing native AdhE ^A267T/E568K mutant

Minimal medium + 10 g/L glucose + 

10 g/L ethanol

Poly(3-hydroxybutyrate) (PHB), 3-hydroxypropionic acid (3-HPA), phloroglucinol (PG)

35.67 g/L PHB (bioreactor level), 0.50 ± 0.01 g/L 3HP (flask), 0.38 ± 0.02 g/L PG (flask)

[14]

E. coli

Introducing the ADH and ALDH from Aspergillus nidulans

M9 + 5 g/L ethanol

Mevalonic acid

\

[71]

E. coli

Introducing exogenous ADH gene from S. cerevisiae and acetaldehyde dehydrogenase (acylating) (Ada) from Dickeya zeae

K3 medium + 10 g/L ethanol

PHB, prenol

1.1 g/L of PHB (flask)

24 mg/L of prenol (flask)

[67]

E. coli

Expressing native AdhE ^A267T/E568K mutant, or co-expressing AdhP and acetylating aldehyde dehydrogenase mutant MhpF_Mut

M9 minimal salt medium containing 10 g/L ethanol

3-HPA

1.66 g/L (flask), 13.17 g/L (whole-cell biocatalysis system)

[76]

Pseudomonas putida

Native pathway accompanied by a heterogeneous expression of acs and eutE

Modified M9 media with 10 g/L of ethanol and 1 g/L of dextrose

Mevalonic acid

0.41 g /g ethanol (flask), 0.32 g /g ethanol (bioreactor)

[69]

Pseudomonas putida

Native pathway accompanied by a heterogeneous expression of Ada

M9 medium containing 1% ethanol

Fatty acid ethyl esters (FAEEs)

1.6 g/L (shake flask), 4.3 g/L (bioreactor)

[27]

Pseudomonas putida

Native pathway accompanied by a heterogeneous expression of Ada and pduP

M9 medium containing 1% ethanol

PHB

1385.34 ± 16.5 mg/L (flask), 4.3 g/L (bioreactor)

[68]

Pseudomonas putida

Native pathway

Digestate medium with about 2 g·L^–1·h⁻¹ ethanol feeding

Medium chain length polyhydroxyalkanoate (mcl-PHA)

6 g/L (bioreactor)

[75]

Corynebacterium glutamicum

Native pathway

LBB + 1% ethanol

VHH, XynA, NEO-2/15

281 mg/L (NEO-2/15, flask)

[26]

Anaerotignum neopropionicum and Clostridium kluyveri

Native pathway

Co-culture, 50 or 90 mM ethanol

Valerate and heptanoate

\

[80]

Yeast

P. pastoris

Native pathway

YNB + 0.5% ethanol

Monacolin J

2.2 g/L (bioreactor)

[19]

P. pastoris

Native pathway

ASMv6 medium with different ethanol concentrations

Recombinant human serum albumin (rHSA)

1.13 mg/g_WCW^b

[79]

P. pastoris

Native pathway

YPE medium containing 0.5% ethanol

Baicalein, Oroxylin A

401.9 mg/L (baicalein, flask), 339.5 mg/L (Oroxylin A, flask)

[21]

Candida utilis

Native pathway

Iron-free salt medium containing 16 g/L or 32 g/L ethanol

Ethyl acetate

\

[82]

Yarrowia lipolytica

Native pathway

Medium containing 2–6 g/L ethanol

Isocitric acid (ICA)

90.5 g/L (bioreactor)

[78]

Yarrowia lipolytica

Native pathway

Basal mineral medium containing less than 2.5 g/L ethanol

α-Ketoglutaric acid

49.0 g/L (bioreactor)

[77]

Other strains

Aspergillus flavus

Native pathway

Potato dextrose broth liquid medium with low-dose ethanol (< 1%)

Aflatoxin

\

[81]