Table 1 S. cerevisiae strains constructed in this study
Strain | Genotype | Source |
|---|---|---|
CEN.PK113-7D | S. cerevisiae CEN.PK113-7D (MATɑ, MAL2-8c SUC2) | EUROSCARF |
MG-0 | S. cerevisiae CEN.PK113-7D Δhis4::TEF1p-Cas9-ADH1t | This study |
MG-1 | MG-0-Site 2:: TEF1p-MGD1-CYC1t | This study |
MG-2 | MG-1ΔDGA1 | This study |
MG-3 | MG-2-Site 5:: GAPp-GAT1-ADH1t | This study |
MG-4 | MG-3-Site 10:: GAPp-ICT1-CYC1t | This study |
MG-5 | MG-4-Site 7:: GAPp-PAH1-PGK1t | This study |
MG-6 | MG-5-Site 1:: GAPp-ACC1-ENO1t-GAPp-ZWF1-PDC1t | This study |
MG-7 | MG-6-Site 11:: TEF1p-MGD1-FBA1t | This study |
MG-8 | MG-7-Site 16:: TEF1p-MGD1- PGK1t | This study |
MG-9 | MG-8-Site 9:: TEF1p-MGD1-ENO1t | This study |