Fig. 3
Sequential dissolution analysis of LPMO-treated Cell I. The figure shows the carbonyl content (A) and the weight-average molecular weight [Mw; (B)] of the fiber fraction in LPMO reactions run for 8 h (left) or 24 h (middle) and of the untreated reference Cell I fiber (right). The fiber fraction was solubilized in DMAc/LiCl [9% (w/v)] solution. At each sampling point, dissolved fibers were removed by filtration and new solvent was added to continue the dissolution. Dissolution times for A and B are therefore expressed in intervals at the bottom of B (note that two slightly different sets of time intervals were used, as explained in Fig. S1). Black bars represent fibers treated with NcAA9C, red bars represent fibers treated with NcAA9C-N, and blue bars represent the untreated Cell I reference fiber. The data for the untreated fiber correspond well to previously established average values [58]. Horizontal dotted lines correspond to values for the reactions with NcAA9C and are meant to facilitate comparisons between identical dissolution times (lines drawn only for fully comparable samples, i.e., samples with exactly the same dissolution time)