Fig. 1
From: Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock
Characterization of ethylene glycol metabolism in E. coli MG1655 and UV-induced mutant E. coli. In LB medium, both EG01 (green) and E. coli MG1655 (blue) exhibited doubling times of 1.58 h−1 and 1.26 h−1, respectively. In M9 medium with ethylene glycol as the sole carbon source, EG01 (black) had a doubling time of 5.22 h.−1, whereas the growth of E. coli MG1655, MG1655 (fucO) overexpressing fucO, and MG1655 (fucO/aldA) overexpressing both fucO and aldA was undetectable. The red line illustrates the change in ethylene glycol consumption by EG01 in M9 (10 g/L EG) medium. Error bars indicated standard error (n = 3)