Fig. 5

The influence of TPA on the growth of ethylene glycol-utilizing E. coli chassis cells. A EG02; (B) EG-BL21(DE3). The E. coli strains, EG02 and EG-BL21(DE3), capable of utilizing ethylene glycol, were separately introduced into M9 medium containing varying concentrations of ethylene glycol, supplemented with equimolar doses of TPA-Na₂ (TPA). 1: 16 mM EG, 16 mM TPA; 2: 80 mM EG, 80 mM TPA; 3: 161 mM EG, 161 mM TPA; 4: 484 mM EG, 484 mM TPA; 5: 806 mM EG, 806 mM TPA; 6: 161 mM EG, 484 mM TPA. The red bars show the initial addition of ethylene glycol in M9 medium, with light red indicating the residual amounts after 72 h of fermentation. The green bars show the initial addition of TPA, with light green indicating the residual amounts after 72 h. The blue bars represent the OD₆₀₀ values of the strain after 72 h of fermentation. Error bars indicated standard error (n = 3)