Fig. 6

Complete metabolic utilization of ethylene glycol in PET degradation product. A Schematic illustration of developing PET enzymatic degradation products as a non-sugar feedstock. B EG02; C EG-BL21(DE3). The Impact of other components, such as MHET, in PET enzymatic degradation products on the growth of EG02 and EG-BL21(DE3). A gradient dilution of the PET degradation product was conducted with M9 medium containing 87 mM equimolar ethylene glycol. Four sets of media were prepared in ratios of 0:5, 1:4, 4:1, and 5:0, and equal amounts of EG02 and EG-BL21(DE3) were inoculated into each. Red bars indicated the addition of PET enzymatic degradation product components in each culture medium. Green bars indicated the addition of M9 components in each culture medium. Light red bars indicated the residual ethylene glycol after 72 h of fermentation. The blue bars represented the OD₆₀₀ value of the strain after 72 h of fermentation. Error bars indicated standard error (n = 3)