Fig. 4

Transcriptome analysis of different strains including WT, OE-Tgglo3, and OE-Tggcs1 in response to lignocellulose. a, b Distribution of significantly up/down-regulated genes (|log₂Fold Change| ≥ 1 and q value < 0.05) of OE-Tgglo3, and OE-Tggcs1 relative to WT. The q value was plotted against the expression fold change of all detected genes within the different groups. Data points in the lower center area of the plots (grey circles) display unchanged or proteins with no significant fold change. Data points in the upper quadrants indicate genes with significant negative (blue circles) and positive (red circles) changes in gene abundances. c GO annotation of differentially expressed genes in different strains. The size of the circle correlates with the number of genes and the color represents the P value. d Hierarchical cluster analysis of the differentially expressed genes in different treatments. The heat map was divided into five regions (R1–R5) according to the differences in gene expression