Fig. 1
From: Engineered reduction of S-adenosylmethionine alters lignin in sorghum
Expression of AdoMetase in sorghum. a Simplified lignin biosynthetic pathway showing the involvement of the two AdoMet-utilizing enzymes COMT and CCoAOMT. Dashed arrows denote multiple steps. AdoCys, S-adenosylhomocysteine. b Construct used for sorghum transformation. Genetic elements in the T-DNA include a selectable marker gene for kanamycin resistance (nptII) placed between a duplicated 35S promoter (p2 × 35S) and a terminator (T35S) from the cauliflower mosaic virus, as well as the AdoMetase synthetic gene located between the sorghum COMT gene promoter (pSbCOMT) and the Agrobacterium tumefaciens nopaline synthase gene terminator (TNOS). c AdoMetase expression in four independent transgenic lines. The level of AdoMetase expression compared to the PP2A gene is shown. First-strand cDNA obtained from wild type (WT) were used as negative control. Values are means ± SD of four biological replicates (n = 4 plants). ND not detected