Fig. 2

Transgene expression in transgenic Chlamydomonas reinhardtii strains after different doses of X-irradiation. The AtPR1-OE1/2 or AtTHI-OE1/2 strains, respectively, harbor an CrRPA70Ap-HSP70Ap-RBCS2p-AtPR1-pOpt_mVenus or CrRPA70Ap-HSP70Ap-RBCS2p-AtTHI2.1-pOpt_mVenus expression construct. Mid-exponential phase cells were subjected to different doses of X-irradiation. XR6, XR12, XR25, and XR50 represent X-rays of 6, 12, 25, and 50 Gy, respectively. The cells were harvested 2 h after X-irradiation. All transcript levels were measured using reverse transcription-quantitative PCR and are shown relative to the mock using CrTUBA1 as an endogenous reference gene. Data represent the mean ± standard error (n = 3). Different letters indicate significant differences at p < 0.05 (one-way analysis of variance, followed by Tukey’s honestly significant difference test)