Fig. 8

Antibacterial activity of wild-type Chlamydomonas reinhardtii cell extracts against Pseudomonas syringae pv. tomato after γ-irradiation. NC, negative control (100% [v/v] dimethyl sulfoxide [DMSO]); PC, positive control (3 µg mL⁻¹ gentamycin in DMSO); Mock or GR1/5/10/20, 100 mg mL⁻¹ control or γ-irradiated cell extract in DMSO. GR1, GR5, GR10, and GR20 represents γ-irradiation at a dose rate of 1, 5, and 10 kGy h⁻¹ for 1 h or 10 kGy h⁻¹ for 2 h, respectively. Cell extracts were prepared via sonication and heating in 100% (v/v) ethanol and used for agar well diffusion assays. The size of the inhibition zone (cm) was calculated by subtracting the well diameter from the width of the clear inhibition area. Data represent the mean ± standard error (n = 6) from two independent experiments. Different letters indicate significant differences at p < 0.05 (one-way analysis of variance, followed by Tukey’s honestly significant difference test)