Fig. 9

Chromatograms of the fatty acids from wild-type Chlamydomonas reinhardtii cell extracts after γ-irradiation obtained through gas chromatography (GC). Mock, control; GR1, GR5, GR10, and GR20 represent γ-irradiation at a dose rate of 1, 5, and 10 kGy h⁻¹ for 1 or 10 kGy h⁻¹ for 2 h, respectively. Cell extracts were prepared via sonication and heating in 100% (v/v) ethanol, methylated to produce fatty acid methyl esters, and used for GC–MS analysis. The numbered GC peaks were selected, quantified, identified using the mass spectral library of the National Institute of Standards and Technology, and then summarized in Table 2