Skip to main content

Table 1 Fresh and dry weights of wild-type and transgenic Chlamydomonas reinhardtii cells after X-irradiation

From: Efficient enhancement of the antimicrobial activity of Chlamydomonas reinhardtii extract by transgene expression and molecular modification using ionizing radiation

Strain

FW (g 0.6 L−1)

DW (g 0.6 L−1)

Mock

XR

Mock

XR

WT

1.527 ± 0.004 a

1.688 ± 0.021 a,*

0.163 ± 0.006 a

0.187 ± 0.007 a,*

AtPR1-OE1

1.527 ± 0.007 a

1.720 ± 0.005 ab,*

0.165 ± 0.008 a

0.210 ± 0.005 ab,*

AtPR1-OE2

1.522 ± 0.007 a

1.740 ± 0.009 bc,*

0.167 ± 0.006 a

0.212 ± 0.006 b,*

AtTHI-OE1

1.527 ± 0.007 a

1.765 ± 0.011 bc,*

0.165 ± 0.006 a

0.212 ± 0.005 b,*

AtTHI-OE2

1.520 ± 0.007 a

1.777 ± 0.008 c,*

0.167 ± 0.006 a

0.215 ± 0.005 b,*

  1. FW: fresh weight; DW: dry weight; WT: wild-type; AtPR1-OE1/2 or AtTHI-OE1/2, transgenic strains overexpressing AtPR1 or AtTHI2.1 proteins; Mock, control; XR, irradiated with X-rays at 6 Gy for the WT or 12 Gy for transgenic strains. Cells were cultivated with an initial optical density of 0.2 measured at 750 nm in 600-mL TAP medium of 1-L flask for 3 days. FW and DW are weights of cells from 600-mL cultures. Data represent the mean ± standard error (n = 6) from three independent experiments. Different letters among strains indicate significant differences at p < 0.05 (one-way analysis of variance, followed by Tukey’s honestly significant difference test). Asterisks indicate significant differences between mock and irradiated samples based on a two-sample independent t-test (p < 0.05)