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Table 2 Changes in the fatty acid composition of wild-type Chlamydomonas reinhardtii cell extracts after γ-irradiation

From: Efficient enhancement of the antimicrobial activity of Chlamydomonas reinhardtii extract by transgene expression and molecular modification using ionizing radiation

Peak no

Peak area (%)

Constituent

Mock

GR1

GR5

GR10

GR20

1

0.22 ± 0.00 ac

0.18 ± 0.00 b

0.21 ± 0.00 a

0.22 ± 0.00 c

0.19 ± 0.00 d

Myristic acid, methyl ester

2

21.90 ± 0.29 a

16.75 ± 0.24 b

16.40 ± 0.18 b

14.76 ± 0.24 c

11.98 ± 0.33 d

Methyl 4,7,10,13-hexadecatetraenoate

3

1.36 ± 0.03 a

0.76 ± 0.01 b

0.87 ± 0.03 c

1.04 ± 0.02 d

0.99 ± 0.02 d

7,10-Hexadecadienoic acid, methyl ester

4

2.22 ± 0.04 a

1.07 ± 0.02 b

1.16 ± 0.03 b

1.07 ± 0.01 b

1.05 ± 0.04 b

7,10,13-Hexadecatrienoic acid, methyl ester

5

3.12 ± 0.05 a

2.66 ± 0.03 b

2.53 ± 0.07 bc

2.49 ± 0.06 bc

2.37 ± 0.10 c

9-Hexadecenoic acid, methyl ester

6

1.56 ± 0.06 ab

1.30 ± 0.02 b

1.50 ± 0.03 ab

1.65 ± 0.02 a

1.67 ± 0.13 a

7-Hexadecenoic acid, methyl ester

7

21.91 ± 0.26 a

19.14 ± 0.32 b

19.16 ± 0.26 b

21.38 ± 0.25 a

19.04 ± 0.41 b

Hexadecanoic acid, methyl ester

8

10.20 ± 0.27 a

7.70 ± 0.25 b

8.17 ± 0.18 b

7.87 ± 0.25 b

12.81 ± 0.73 c

Methyl 5,9,12-octadecatrienoate

9

6.90 ± 0.22 a

4.92 ± 0.09 b

6.05 ± 0.31 c

5.83 ± 0.17 c

4.93 ± 0.14 b

9,12-Octadecadienoic acid, methyl ester

10

27.17 ± 0.97 a

26.97 ± 0.40 a

24.37 ± 0.24 b

21.94 ± 0.34 c

23.13 ± 0.58 bc

9,12,15-Octadecatrienoic acid, methyl ester

11

2.20 ± 0.05 a

2.11 ± 0.05 ab

1.96 ± 0.03 bc

2.01 ± 0.05 bc

1.84 ± 0.04 c

9-Octadecenoic acid, methyl ester

12

1.23 ± 0.05 a

2.40 ± 0.04 bd

2.11 ± 0.03 b

3.08 ± 0.19 c

2.55 ± 0.05 d

Methyl stearate

13

N.D. a

7.56 ± 0.13 b

7.86 ± 0.18 b

7.94 ± 0.27 b

7.77 ± 0.26 b

n-Hexadecanoic acid (palmitic acid)

14

N.D. a

5.35 ± 0.52 b

6.32 ± 0.27 bc

7.15 ± 0.18 cd

7.63 ± 0.21 d

9,12,15-Octadecatrienoic acid (linolenic acid)

15

N.D. a

0.74 ± 0.08 b

0.91 ± 0.08 bc

1.01 ± 0.02 c

1.40 ± 0.06 d

9-Octadecenoic acid (oleic acid)

16

N.D. a

0.39 ± 0.02 b

0.43 ± 0.03 b

0.56 ± 0.01 cd

44 ± 0.03 d

Octadecanoic acid (stearic acid)

  1. Mock, control; GR1, GR5, GR10, and GR20 represent γ-irradiation at a dose rate of 1, 5, and 10 kGy h−1 for 1 h or 10 kGy h−1 for 2 h, respectively; N.D., not detected. Data represent the mean ± standard error (n = 6) from three independent experiments. Different letters among groups indicate significant differences at p < 0.05 (one-way analysis of variance, followed by Tukey’s honestly significant difference test)
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Biotechnology for Biofuels and Bioproducts

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