Fig. 1

Schematic of engineered metabolic pathways for hexanoic acid and olivetolic acid biosynthesis in S. cerevisiae. CoA biosynthesis pathway (orange), rBOX pathway (blue), FAB pathway (purple), olivetolic acid biosynthesis pathway (green), peroxisomal β-oxidation (gray). Upregulated genes (black) and knocked out genes (red) are illustrated. rBOX—reverse β-oxidation; FAB—fatty acid biosynthesis; CoA—coenzyme A; Ec—Escherichia coli; Cn—Cupriavidus necator; Ca—Clostridium acetobutylicum; Td—Treponema denticola; Cs—Cannabis sativa; gpd2—glycerol 3-phosphate dehydrogenase 2, adh1-5—alcohol dehydrogenase 1–5; bktB—β-ketothiolase; paaH1—3-hydroxyacyl-CoA dehydrogenase; crt—short-chain enoyl-CoA hydratase; ter—trans-2-enoyl-CoA reductase; faa2—medium-chain fatty acyl-CoA synthetase; fusFAS*—mutant fused fatty acid synthase; TEs—thioesterases; OLS—olivetol synthase; OAC—olivetolic acid cyclase