Fig. 3
FAA2 knockout prevents degradation of reverse β-oxidation pathway-derived hexanoic acid in S. cerevisiae. A Effect of FAA2 deletion on hexanoic acid degradation after 48 h and 72 h in rBOX pathway strain. B Effect of FAA2 deletion on glucose consumption in rBOX pathway strain. C MCFA output after 96 h following expression of fusFASIAGSMWFY in the ∆faa2 rBOX strain (yrBOX1). C6-FA—hexanoic acid; fusFAS—fused fatty acid synthase; rBOX—reverse β-oxidation. Data represent mean ± s.d.; n = 3 biologically independent samples. Statistical analysis was performed using the two-tailed unpaired t-test. p > 0.05 = ns (not significant); p < 0.05 = *; p < 0.01 = **; p < 0.001 = ***; p < 0.0001 = ****