Table 1 List of strains constructed and used in this study

S. cerevisiae

SHY24

FAS^WT

MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; ∆faa2

[36]

SHY34

fas^null

SHY24 ∆fas1; ∆fas2

[36]

GDY27

GDY27

MATa his3-Δ1 leu2-3,112 trp1-289 MAL2-8C SUC2; adh1∆::loxP; adh2∆::LEU2; adh3∆::loxP; adh4∆::loxP; adh5∆::loxP; ∆gpd2; ura3∆::P_HHF1-^CnbktB-tENO1, P_CCW12-^CnpaaH1-tIDP1, P_ENO2-^Cacrt-tPGK1, P_TDH3-^Tdter-tADH1, kanMX4

[25]

KSY13

yrBOX1

GDY27 ∆faa2

This study

KSY23

adh6∆::^EccoaA

yrBOX1 adh6∆::P_PGK1-^EccoaA-tSSA1

This study

KSY22

yrBOX2

yrBOX1 leu2∆::P_PGK1-^EccoaA-tSSA1, natNT2

This study

KSY26

yrBOX3

yrBOX2 P_fms1∆::P_ADH1

This study

E. coli

DH10ß

F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(Str^R) nupG

New England Biolabs