Table 2 List of plasmids constructed and used in this study

pRS313

pRS313

CEN4ARS6, Amp^R, HIS3

Addgene

FWV132

pRS313-fusFAS^WT

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1-FAS2-tFAS2

[36]

ALSV7

pRS313-fusFAS^RK

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^RK-FAS2-tFAS2

Lab stock

ALSV9

pRS313-fusFAS^RKFY

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^RK-FAS2^FY-tFAS2

Lab stock

ALSV11

pRS313-fusFAS^IAGSMWFY

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^IA-FAS2^GSMWFY-tFAS2

Lab stock

ALSV13

pRS313-fusFAS^IARKGSMWFY

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^IARK-FAS2^GSMWFY-tFAS2

Lab stock

KSV8

pRS313-fusFAS^IAGS

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^IA-FAS2^GS-tFAS2

This study

KSV9

pRS313-fusFAS^IARKGS

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^IARK-FAS2^GS-tFAS2

This study

KSV10

pRS313-fusFAS^IAGSMW

CEN4ARS6, Amp^R, hphNT1, P_TDH3-FAS1^IA-FAS2^GSMW-tFAS2

This study

KSV30

pRS313-fusFAS^IAGSMWFY

CEN4ARS6, Amp^R, kanMX4, P_TDH3-FAS1^IA-FAS2^GSMWFY-tFAS2

This study

KSV55

pRCC-N-ADH6

2µ, Amp^R, natNT2, P_ROX3-^Spcas9-tCYC1, pSNR52-[ADH6]-sgRNA-tSUB4

This study

SHV42

pRCC-N-FAA2

2µ, Amp^R, natNT2, P_ROX3-^Spcas9-tCYC1, pSNR52-[FAA2]-sgRNA-tSUB4

[3]

KSV75

pRCC-H-P_FMS1

2µ, Amp^R, hphNT1, P_ROX3-^Spcas9-tCYC1, pSNR52-[P_FMS1]-sgRNA-tSUB4

This study

pVS5_4

^EccoaA template

2µ, natNT2, Amp^R, ^EccoaA

[24]

KSV52

^EccoaA expression plasmid

2µ, Kan^R, hphNT1, P_PGK1-^EccoaA-tSSA1

This study

KSV53

^EccoaA integration plasmid

Kan^R, LEU2 5’ HR, P_PGK1-^EccoaA-tSSA1, natNT2, LEU2 3’ HR

This study

KSV66

P_ADH1-FMS1

2µ, Kan^R, URA3, P_ADH1-FMS1-tTDH1

This study

KSV67

P_HSP26-FMS1

2µ, Kan^R, URA3, P_HSP26-FMS1-tTDH1

This study

KSV68

P_TEF1-FMS1

2µ, Kan^R, URA3, P_TEF1-FMS1-tTDH1

This study

KSV74

OLS-OAC

2µ, Kan^R, URA3, P_TEF2-^CsOLS-tADH1, P_TEF1-^CsOAC-tHXK2

This study

pRS41H

EV

CEN4ARS6, Amp^R, hphNT1

[37]

pRS41K

kanMX4 template

CEN4ARS6, Amp^R, kanMX4

[37]

KSV48

EV (pRS313-kanMX4)

CEN4ARS6, Amp^R, kanMX4

This study

SiHV005

EV

2µ, Kan^R, URA3

Lab stock

SiHV010

EV

2µ, Kan^R, hphNT1

Lab stock