Table 3 Oligonucleotides used for plasmid construction and strain engineering

SHP110

TGACTGCGATGATAGGAGG

Forward primer for amplifying ∆faa2 locus in SHY24

SHP222

GTGCACCAAGTCAAGTTACG

Reverse primer for amplifying ∆faa2 locus in SHY24

ALSP7

GTTGTGTTCTACAAAGGTATGAC

Forward primer for insertion of R1834K mutation in FAS1

ALSP8

GTCATACCTTTGTAGAACACAAC

Reverse primer for insertion of R1834K mutation in FAS1

ALSP13

GGCAATTACTGTATTATTCTTCGCCGGTGTTCGTTGTTACG

Forward primer for insertion of I306A mutation in FAS1

ALSP14

CGTAACAACGAACACCGGCGAAGAATAATACAGTAATTGCC

Reverse primer for insertion of I306A mutation in FAS1

ALSP15

GTTCTGGTTCTAGTTGGGGTGGTGTTTC

Forward primer for insertion of G1250S and M1251W mutations in FAS2

ALSP16

GAAACACCACCCCAACTAGAACCAGAAC

Reverse primer for insertion of G1250S and M1251W mutations in FAS2

KSP20

GTTCTGGTTCTTCTATGGGTGGT

Forward primer for insertion of G1250S in FAS2

KSP21

ACCACCCATAGAAGAACCAGAAC

Reverse primer for insertion of G1250S in FAS2

KSP37

TTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATCAGCGACATGGAGGCC

Forward primer for amplification of kanMX4 cassette with overhangs for pRS313 to replace marker (hphNT1 or HIS3)

KSP38

TCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGATCCGGACACTGGATGGCGGC

Reverse primer for amplification of kanMX4 cassette with overhangs for pRS313 to replace marker (hphNT1 or HIS3)

KSP72

GAGGAAGAAATTCAACACAACAACAAGAAAAGCCAAAATCGTGAGTAAGGAAAGAGTGAG

Forward primer for amplifying ^EccoaA cassette from KSV52 with overhangs for ADH6 integration locus

KSP73

AAAGAAAGGAGCTACATTTATCAAGAGCTTGACAACATAAAATTAAAGTAGCAGTACTTC

Reverse primer for amplifying ^EccoaA cassette from KSV52 with overhangs for ADH6 integration locus

KSP88

ACGGTTCAATCGCAATTTCTCCGGAAAGTGCAGTAGCAACTGTAGCCCTAGACTTGATAG

Forward primer for amplifying P_ADH1 from S. cerevisiae genome with overhangs for P_FMS1 locus

VSP375

TTTGGCTGGTGAAACTGTATTCATTGTATATGAGATAGTTGATTGTATGC

Reverse primer for amplifying P_ADH1 from S. cerevisiae genome with overhangs for P_FMS1 locus