Valorization of milling byproducts and ergot-sclerotia-contaminated rye via clostridial ABE fermentation

Table 1 Solvent production of clostridia strains on medium with milling byproducts as substrate

Substrate	strain	Solvent production [g/L]
		Acetone			Butanol			Ethanol
15% WRD	C. beijerinckii NCIMB 8052	4.4	±	0.4	13.1	±	0.7	N. Q
	C. diolis DSM 15410	2.2	±	0.1	7.2	±	0.2	N. Q
	C. saccharobutylicum DSM 13864	6.4	±	0.8	12.8	±	1.9	N. Q
	C. saccharoperbutylacetonicum N1-504	7.0	±	2.8	10.0	±	4.0	N. Q
15% RSF	C. beijerinckii NCIMB 8052	5.3	±	0.4	13.5	±	1.0	N. Q
	C. diolis DSM 15410	5.2	±	0.6	13.3	±	1.9	N. Q
	C. saccharobutylicum DSM 13864	5.5	±	1.0	9.8	±	1.7	N. Q
	C. saccharoperbutylacetonicum N1-504	0.8	±	0.0	9.9	±	1.7	N. Q
15% WB	C. beijerinckii NCIMB 8052	1.4	±	0.0	7.1	±	0.9	N. Q
	C. diolis DSM 15410	1.3	±	0.1	4.1	±	0.1	N. Q
	C. saccharobutylicum DSM 13864	4.2	±	3.2	7.4	±	0.4	N. Q
	C. saccharoperbutylacetonicum N1-504	1.6	±	0.0	2.2	±	0.1	N. Q
15% RB	C. beijerinckii NCIMB 8052	3.5	±	0.6	9.9	±	0.2	N. Q
	C. diolis DSM 15410	1.6	±	0.1	5.4	±	0.6	N. Q
	C. saccharobutylicum DSM 13864	1.8	±	0.2	1.8	±	0.4	N. Q
	C. saccharoperbutylacetonicum N1-504	0.4	±	0.0	2.3	±	0.2	N. Q
15% ER	C. beijerinckii NCIMB 8052	3.3	±	0.1	11.1	±	0.4	N. Q
	C. diolis DSM 15410	3.7	±	0.2	11.3	±	0.6	N. Q
	C. saccharobutylicum DSM 13864	6.6	±	0.7	11.6	±	0.8	N. Q
	C. saccharoperbutylacetonicum N1-504	3.3	±	0.1	10.3	±	0.4	N. Q

The strains were incubated 72 h at 34 °C in GM plus 15% of respective carbon source. The fermentation products acetone and butanol were measured using GC-FID. Ethanol was found, but concentrations were low and not quantified (N.Q.). The experiment was performed in duplicate

ISSN: 2731-3654