Fig. 7

Mevalonate production by KO_MvaES, during six parallel fed-batch fermentations with defined \(\mu\)_set values. Values normalised to t = 0, for the beginning of steady-state conditions after batch phase, once feed has begun. A Specific growth rate of fed-batch reactions. B Specific product formation rate (q_p) of fed-batch reactions. C Specific substrate consumption rate (q_s) of fed-batch reactions. D Amount of glucose present in the culture medium. E Table containing the values, root mean squared error (RMSE) and correlation coefficient (R²) of \(\mu\), q_p and q_s for each \(\mu\)_set, obtained from panels A-C, and titre after 24 h of feeding. Blue circles: \(\mu\)_set = 0.4 h⁻¹, orange squares: \(\mu\)_set = 0.35 h⁻¹, grey diamonds: \(\mu\)_set = 0.3 h⁻¹, yellow triangles: \(\mu\)_set = 0.25 h⁻¹, light blue squares: \(\mu\)_set = 0.2 h⁻¹, green circles: \(\mu\)_set = 0.15 h⁻¹