Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’

Table 2 Growth of xylose-fermenting S. cerevisiae CT2 Pro and NKSW strains on various carbon sources with/without nutrient supplement

Media	Liquid ratios	CT2 pro growth	NKSW growth
Hydrolysate	100	−	−
Hydrolysate + Water	50:50	+	−
Hydrolysate + Water	20:80	+	+
Hydrolysate + Juice	50:50	+ +	+ +
Hydrolysate + Water + Juice	25:25:50	+ +	+ +
Juice	100	+ + + +	+ + + +
Hydrolysate + Water + urea^a	50:50	−	−
Hydrolysate + Water + CSM^b	50:50	−	−
Hydrolysate + Water + CSM^b + sodium sulfite^c	50:50	−	−
Hydrolysate (pH 5)	100	+ +	−
Hydrolysate + Water (pH 5)	50:50	+ + + +	+ + + +
Hydrolysate + Juice (pH 5)	50:50	+ + + +	+ +

Cells were rated for growth: strong growth (+ + + +), medium growth (+ +), slight growth ( +) and no growth (−)
^aUrea (5 g/l)
^bComplete amino acids mixture (0.67 g/l)
^cSodium sulfite (12.5 mM/1.57 g/l)

ISSN: 2731-3654