Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’

Table 3 Kinetics of engineered S. cerevisiae strains CT2-Pro, NKSW7-1 and commercial strain on the transgenic oilcane hydrolysate and an equal blend of hydrolysate and juice used as media without detoxification or nutrient supplementation

S. cerevisiae strains	Carbon Source^a	Total sugar (g/l)	Sugar consumed^b (g/l)	Ethanol titer (g/l)	Biomass yield (g/g)	Ethanol yield (g/g)	SEP^c (g/g^.h)	VEP^d (g/l^.h)	SSC Rate^e (g/g^.h)
CT2-Pro	Hydrolysate	131.29 ± 0.01	88.54 ± 6.50	10.73 ± 1.42	0.027 ± 0.002	0.121 ± 0.007	0.026 ± 0.004	0.06 ± 0.008	0.22 ± 0.01
CT2-Pro	50:50 H:J	185.89 ± 0.38	95.11 ± 3.32	13.66 ± 0.13	0.025 ± 0.001	0.181 ± 0.003	0.100 ± 0.009	0.19 ± 0.001	0.55 ± 0.04
NKSW7-1	Hydrolysate	132.02 ± 0.48	0	0	ND^f	ND	ND	ND	ND
NKSW7-1	50:50 H:J	148.63 ± 0.08	63.35 ± 0.55	0.92 ± 0.01	0.035 ± 0.003	0.026 ± 0.001	0.011 ± 0.001	0.01 ± 0.001	0.31 ± 0.03
Commercial strain	Hydrolysate	115.84 ± 0.26	0	0	ND	ND	ND	ND	ND
Commercial strain	50:50 H:J	152.67 ± 4.41	82.24 ± 0.32	20.33 ± 0.18	0.044 ± 0.009	0.273 ± 0.013	0.067 ± 0.011	0.21 ± 0.001	0.24 ± 0.05

^aCarbon source consisted of either undiluted hydrolysate (H) or an equal blend of hydrolysate and juice (H:J)
^bTotal sugar consumption includes consumption of only fermentable sugars (arabinose is not included) till 168 h
^cSpecific ethanol productivity
^dVolumetric ethanol productivity
^eSpecific sugar consumption rate
^fNot determined—no growth was observed for NKSW7-1 and commercial yeast strain in hydrolysate cultures

ISSN: 2731-3654