Fig. 2

Optimization of acid cleavage conditions for fusion protein DAMP4–DPS–Kiadin. A Tricine–SDS–PAGE showing protein bands of DAMP4–DPS–Kiadin versus DAMP4–DPIPDPS–Kiadin after cleavage with HCl for different temperatures (60 °C, 75 °C) and different hours (24 h, 48 h), respectively. B Inhibitory activity of acid cleavage products of DAMP4–DPS–Kiadin obtained under different temperatures 75 °C, 78 °C, 81 °C, 84 °C, 87 °C and 90 °C for 1–6 h on E. coli K88. The inhibitory activity of DAMP4–DPS–Kiadin without any treatment was set as control. C Surviving colonies of E. coli K88 treated with acid cleavage products of DAMP4–DPS–Kiadin at 2 h at different temperatures (75 °C, 78°Cand 81 °C), the inhibitory activity of DAMP4–DPS–Kiadin without any treatment was set as control. D Tricine–SDS–PAGE showing protein bands of DAMP4–DPS–Kiadin cleaved in HCl and H₃PO₄ for 4 h and 24 h, respectively