Fig. 4
Kinetics of lipid extraction from A. protothecoides using monophasic ethanol:hexane:water, 1:0.41:0.04 vol/vol/vol, co-solvent system with 1g:78mL biomass:solvent ratio after PEF-treatment (A) without any incubation or (B) with a 24 h of incubation after PEF-treatment (B). No kinetics extraction was performed from untreated biomass given the insignificant yields (lipid yield from extraction performed on small volumes of control biomass were in the range of 0.8–1.81%). The dashed lines represent the non-linear fitting performed by Origin software using the one-phase exponential decay function with a time constant parameter expressed as C(t) = Cw + CD(1- exp(-kdt)) as detailed in paragraph 3.2. The straight line in the yellow zone indicate the total lipid content of the biomass as determined by chloroform:methanol extraction. Results are the average ± std from three independent cultivations with internal duplicates