Fig. 5

Observation of lipid distributions after PEF-treatment. Cells were fixed either immediately or 24 h after PEF treatment and stained with Nile-Red. The images are the bright field and the overlay of GFP (green) and RFP (red) channels. The bars are 10 μm. A The emission shifting from both GFP and RFP to mostly FRP could indicate an increase of polarity and mixture of lipids with more polar cell components