Fig. 6
Biphasic lipid extraction from wet A. protothecoides after PEF-treatment with 0.25 MJ/kgdw or 1.5 MJ/kgdw. The solvent mixture used was a biphasic ethanol:hexane:water co-solvent system 1:6:0.4 vol/vol/vol with a ratio of 37 mL per 1 g of dry biomass. The straight line in the yellow zone indicate the total lipid content of the biomass as determined by chloroform:methanol extraction. Extraction took place either immediately or after 24-h incubation after PEF-treatment. Results are the average + std of three independent experiments